Biopreparation RIAL and method for feeding various groups of animals, poultry and bees

ABSTRACT

The RIAL biopreparation contains free and fixed amino acids, lipids, macronutrients and microelements, compounds of non-metals, carbohydrates and moisture in the following proportions, 30-40, 30-55, 0.01-2, 2-6, 0.5-1.5, 8-12, 0.001-0.1, 4-10 wt.-%, respectively. The method for feeding various groups of animals, poultry and bees involves introducing the RIAL biopreparation into their basic ration. The dose of the preparation used in animal breeding is 10-100 mg/kg of body weight. In poultry farming, the dose is 330-625 g/t of feed. In apiculture, the RIAL preparation is introduced into sugar syrup in a dose of 50-150 mg/l of syrup.

FIELD OF ART

The present invention relates to applied biotechnology, ecology,processing of animal and biological raw materials, animal breeding and,more specifically, it relates to a biopreparation of a wide range ofapplication and to its utilization as a food addition.

BACKGROUND OF THE INVENTION

At present, anthropogenic abuse of nature has brought about an overallecological pollution which resulted in a reduction of natural resistanceof the animal organism and spreading of animal diseases.

In this connection, great importance should be attached to the problemof improving the protective forces of the animal organism including theuse of biologically active preparations.

The stimulating preparations resorted to in animal breeding contributeto raising the natural resistance of the organism, and to acceleratedgrowth and development of animals.

Biologically active preparations added to fodder increase the biotonusof the organism, normalize metabolism, improve the physiologicalcondition and reproductive functions of animals and poultry.

Known in the prior art is a number of biological preparations producedby hydrolysis (FRG Application No. 3128503, A61K35/26; U.S. Pat. No.3,898,329, A61K37/00; Application of France No. 2574630, A23K1/10).

The above-cited preparations have a number of inherent disadvantages,namely: insufficient stimulating effect, narrow range of applicationand, limited group of treated agricultural animals. In addition, itshould be noted that these preparations are available in a liquid formwhich involves certain difficulties in their administration.

Known in the prior art is a biostimulant "Splenivita" prepared fromanimal spleen (USSR Inventor's Certificate No. 1695869, A23K1/00, 1991).However, this stimulant features an insufficient activity and acomparatively narrow field of action.

Also known in the prior art is an albuminous hydrolysate prepared fromwaste of fur and leather manufacture (USSR Inventor's Certificate No.1699401, A23J1/10, 1991). From the viewpoint of its composition, saidhydrolysate is closest to the claimed preparation though is less activeand has a smaller range of application.

As for the field of application of the claimed preparation, it ispertinent to note the following.

There is a known method for fattening broiler chickens (USSR Inventor'sCertificate No. 1340715, USSR Inventor's Certificate No. 1340715,A23K1/00, 1987) wherein the food ration is enriched by introducingaminobutyric acid in the capacity of a biostimulant.

A disadvantage of this method lies in its low efficiency.

Also known is a method of poultry feeding (USSR Inventor's CertificateNo. 1530163, A23K1/16, 1989) which involves enriching the food rationwith a biostimulant in the form of egg mass. However, this method islikewise poorly effective.

Thus, the problem of developing an effective biopreparation used inanimal breeding as a biostimulating feed addition still remains of vitalimportance.

DISCLOSURE OF THE INVENTION

The main object of the disclosed group of inventions resides inproviding a biopreparation featuring the property of a highly-effectivemultipurpose biostimulant and in developing a method of using saidbiopreparation in the food ration of various groups of animals, poultryand bees.

This object is attained by providing a biopreparation RIAL developed bydestructive conversion of inedible biological materials of differentorigin, containing free and fixed amino acids, lipids, macronutrients,microelements, non-metal compounds, carbohydrates and moisture in thefollowing proportions, wt.-%:

    ______________________________________                                        Amino acids:                                                                  ______________________________________                                        free              30-40                                                       fixed             30-55                                                       Lipids            0.01-2                                                      Macronutrients    2-6                                                         Microelements     0.5-1.5                                                     Non-metal compounds                                                                              8-12                                                       Carbohydrates     0.001-0.1                                                   Moisture           4-10                                                       ______________________________________                                    

Amino acids are a product of albuminolysis wherein the term "free aminoacids" is understood as natural 1-aminoacids while fixed amino acids asthe fragments of hormones, # enzymes and other lower peptides.

The macronutrients in the RIAL biopreparation are represented by iron,calcium, sodium, potassium, phosphorus, magnesium, sulfur, chlorine,while microelements, by manganese, zinc, copper, antimony, arsenic, tin,molybdenum, bismuth, etc.

The non-metal compounds contain fats, purines, pyrimidines, andfragments of polysaccharides while carbohydrates are constituted bymonosugars.

The biopreparation thus comprises organic and inorganic parts. Theorganic part includes free and fixed amino acids, lipids and otherorganic compounds (purines, pyrimidines, fragments of polysaccharides inthe form of oligo- and monosaccharides). The inorganic part comprisesmetals and non-metals which can be divided into macro andmicro-elements.

The newest drying equipment is capable of producing RIAL biopreparationwith a lower moisture content.

The biopreparation is, essentially, a hygroscopic powder with a slightspecific odor, varying in color from light-yellow to brown.

The RIAL is nontoxic, readily assimilated and easily mixed with waterand feed.

The RIAL biopreparation is a natural complex of organic and inorganiccompounds containing the balanced quantities of natural 1-amino acids,monosugars, lower peptides, carbohydrates, lipides, fragments ofvitamins, hormones, enzymes, a set of macronutrients and microelements.Actually, all these substances form the living matter which, afterdecomposition of biological systems, is again split back into thesesubstances. The RIAL preparation contains an optimum proportion ofbiologically active components.

The technical result of using the RIAL biopreparation in the capacity ofa feed addition increases the biotonus of the organism and normalizesmetabolism which enhances working capacity and endurance, naturalresistance and productivity of animals, poultry and bees.

The vital importance of the present invention is due to the fact thatthe RIAL biopreparation can be produced from nonstandard animal rawmaterials whose utilization relieves harmful ecological load onenvironment.

The process of producing the RIAL biopreparation involves the use ofwhole or disintegrated carcasses of weak, frost-bitten, sick, perishedand other categories of animals, or some other biological material (e.g.blood). The processed protein loses completely its specific features andantigenic properties. All initial feedstuff is converted into a usefulproduct without any waste and renders this process ecologically clean.

The production process includes the following stages:

1. Homogenization (if necessary) of source materials--protein-containingwaste of animal husbandry.

2. Acidic hydrolysis of source materials in 5-20% sulfuric acid at90°-110° C. for 5-15 h.

3. Filtering of acidic hydrolysate.

4. Alkaline hydrolysis of residue non-dissolved in acid with calciumoxide at 95-105 C. for 3-5 h.

5. Combining and mixing the acidic and alkaline hydrolysates.

6. pH correction (if necessary).

7. Filtration.

8. Concentration of filtrate.

9. Drying of concentrated solution.

10. Packing.

When the RIAL biopreparation is produced from blood, the homogenization,alkaline hydrolysis and, correspondingly, subsequent filtration stagesare left out from the production process.

The Table 1 below gives the composition of the RIAL biopreparationproduced by the above method from animal raw materials (rabbitcarcasses,) and biological materials (cattle blood).

                  TABLE 1                                                         ______________________________________                                        Composition of RIAL Biopreparation                                            Produced from Various Raw Materials                                                            Blood,  Rabbit, car-                                         Ingredients      wt.-%   casses, wt.-%                                        ______________________________________                                        Amino Acids      65-67   65-67                                                Lipids           2       1.9                                                  Macronutrients   2       2.3                                                  Microelements    0.5     0.8                                                  Non-metal compounds                                                                             8-11   9.5                                                  Carbohydrates    0.05    0.1                                                  Moisture         4.5-4.8 4.5                                                  ______________________________________                                    

Table 2 gives a comparative analysis of amino acid composition of thebiopreparation made of animal carcasses than cannot be processed intofood products, from blood and dry blood.

                  TABLE 2                                                         ______________________________________                                        Content of Amino Acids in the                                                 RIAL Biopreparation                                                           Amino acids                                                                            Waste, mg % Blood, mg %                                                                              Dry blood, mg %                               ______________________________________                                        Aspartic acid                                                                          8.044       9.56       9.12                                          Threonine                                                                              3.372       0.79       1.43                                          Serine   3.546       1.68       2.1                                           Glutamic acid                                                                          7.3         3.97       4.86                                          Proline  3.39        --         --                                            Glycine  3.082       2.35       2.33                                          Alanine  5.774       3.23       3.39                                          Valine   5.955       1.48       1.68                                          Methionine                                                                             1.024       0.27       0.61                                          Isoleucine                                                                             0.833       0.37       0.42                                          Leucine  8.886       2.24       3.53                                          Tyrosine 1.075       6.87       6.92                                          Phenylalanine                                                                          4.674       --         --                                            Lysine   6.864       1.32       1.87                                          Histidine                                                                              5.706       2.47       2.2                                           Arginine 4.276       3.45       3.23                                          Cysteine 0.712       --         --                                            ______________________________________                                    

BEST MODE OF CARRYING OUT THE INVENTION

The RIAL biopreparation of the above-mentioned composition is used as afeed addition to improve the working capacity and endurance of horses,to increase natural resistance, productivity and reproductive capacitiesof pigs, sheep, fur farming animals, poultry and bees.

In horse-breeding, the preparation is mixed with concentrated feeds oris added to cooked grain and given once daily at the rate of 30 mg/kg ofbody weight.

If the preparation is administered in excess of two months, it ispracticable that it be given by ten-daily courses with 10-day intervalsor every other day.

In pig breeding, the preparation is fed daily as a part of regularration in the following doses:

sows--25-40 mg/kg of body weight 15 days before and 30 days afterfarrowing;

piglets--30-50 mg/kg of body weight in the course of a month.

In sheep breeding, the preparation is fed to pregnant ewes in a mixturewith concentrated feeds daily in the morning in the course of 30 daysbefore lambing in a dose of 30 mg/kg of body weight.

The lambs are given the preparation individually, 2-3 h after the birthand in the course of the first month of life by forced administration ina dose of 10 mg/kg of body weight in the form of a solution in cleanboiled water from a Janet syringe, daily or every other day.

After the first month the lambs are given the RIAL biopreparation mixedwith concentrated feeds, daily for 30 days in a dose of 10 mg/kg of bodyweight.

The tupping rams are given the preparation individually in the form of asolution in clean boiled water in the course of 50-55 days beforeinsemination through a Janet syringe and a rubber tube in a dose of 10mg/kg of body weight.

Within the period of intensive sexual exploitation of tupping rams, thedose may be brought to 30 mg/kg of body weight.

The dose of the preparation given to rams with a low sexual potency isincreased to 50 mg/kg while after restoring the reproductive function,it is reduced to the normal limit, i.e. 30 mg/kg of body weight.

When tupping rams are subjected to mass treatment before artificialinsemination, the biopreparation may be administered in a mixture withconcentrated feeds provided the animals are equalized in groups by thebody weight.

In fur farming, the biopreparation is given in a mixture with theregular ration.

Minks--ten-daily, a dose of 50 mg/kg of body weight:

male minks in the pre-rutting and rutting periods;

female minks--in the pre-rutting and rutting periods, during pregnancyand nursing of cubs;

young minks--from weaning to slaughtering.

Polecats--ten-daily, a dose of 100 m/kg of body weight:

male polecats--in the pre-rutting and rutting periods;

female polecats and other sex-age groups from February 1 to June 1.

Sables--ten-daily, a dose of 100 mg/kg of body weight:

male sables--in the pre-rutting and rutting periods;

female sables and other sex-age groups from January 1 to June 1.

In poultry farming, the preparation is administered together withformula feeds, most efficiently in the composition of low-nutrient feedmixtures. The RIAL biopreparations should be introduced into formulafeeds by steps: first mixing them with 0.5-1 kg of formula feed, thenwith 5-10 kg of formula feed, and mixing this amount of feed with 1 t offormula feed.

The daily doses of the RIAL biopreparation are:

broiler chickens--330-550 g per 1 t of feed within the first or entiregrowing period;

laying hens--375-625 g per 1 t of feed within the productive period.

In apiculture, the preparation is mixed with syrup for feeding thecolonies in spring and autumn. First, the preparation is dissolved in asmall amount of water, poured into the syrup and mixed. The syrup isgiven in warm weather in upper and side feeders.

In spring, the preparation is used as a stimulating addition to step upthe growth of bee colonies--a liter of syrup per colony at 3-5 dayintervals, 150 m/l of the RIAL biopreparation.

In autumn, the preparation is used to improve wintering of bee coloniesin case of scarcity of feed reserves. For this purpose, the sugar syrupis mixed with 50 mg/l of the RIAL biopreparation.

When used in recommended doses, the RIAL biopreparation causes no sideeffects and complications. There are no contraindications.

The advantages and specific features of the present group of inventionswill be understood from the following description of the effect of theRIAL biopreparation on the physiological parameters of agriculturalanimals, poultry and bees as illustrated by examples below.

EXAMPLE 1 Effect of Various Dosages of the RIAL Biopreparation onProductive Capacities of Sheep

A series of scientific and economic and physiological experiments hasbeen conducted at the physiological station of the "Temnolesskoye" farmof VNIIOK (All-Union Research Institute of Sheep and Goat Breeding) onmerino sheep of the Stavropol breed. The groups of pregnant ewes, lambsand tupping rams have been formed on the principle of analog pairs,taking in account the pregnancy time, time of delivery and body weightof lambs, also body weight and reproductive ability of sires.

In each experiment, the animals were fed identically in accordance withthe feeding standards for individual sex-age groups.

The RIAL biopreparation was administered to pregnant ewes, lambs andtupping rams individually in morning hours.

                  TABLE 3                                                         ______________________________________                                        Body Weight of Lambs New-Born from Ewes which                                 Received the RIAL Preparation 30 days before Lambing                                     Body           Body weight                                                    weight RIAL    of new-born                                                No. of                                                                              of ewes, dose,   lambs, kg                                       Animal group                                                                           ewes    kg       mg/kg singles, kg                                                                           twins, kg                             ______________________________________                                        1st exper.                                                                             20      61.8     10    5.2     3.7                                   2nd exper.                                                                             20      61.6     30    5.7     3.6                                   Control  20      60.5     --    5.1     3.2                                   ______________________________________                                    

It follows from Table 3, that feeding the pregnant ewes before lambingwith the RIAL biopreparation influences the body weight of lambs whichtestifies that the RIAL biopreparation is an efficient exogenic plasticmaterial contributing to the weight gain of sheep particularly at theembryonic development stage.

The same is confirmed by the data of Table 4 showing that the plasticmaterial of the RIAL biopreparation can be carried with blood into ewe'smilk and passed over to the offsprings in the form of additionalalbuminous, nitrous, fatty and mineral substances, and be reliablyincreased in the blood of young sheep due to its dosed administrationfrom the first days after the birth.

                  TABLE 4                                                         ______________________________________                                        Chemical Composition of Milk of Ewes                                          Fed with Various Doses of RIAL 30 Days before Lambing                                   RIAL dose, mg/kg                                                    Composition, %                                                                            10          30     Control, %                                     ______________________________________                                        Total nitrogen                                                                            0.91        1.15   0.84                                           Albumin     5.55        6.19   5.29                                           Ash         1.03        1.11   0.97                                           Calcium     0.20        0.21   0.18                                           Phosphorus  0.12        0.13   0.12                                           Fat         5.8         6.6    5.2                                            Total sulfur                                                                              0.04        0.04   0.04                                           Sugar, g/100 ml                                                                           5.8         5.11   4.92                                           ______________________________________                                    

In the dosed administration of the RIAL biopreparation to single lambsin the course of 1 month after the birth, the figures of body weightgain within this month in experimental groups were 4.1 kg higher thanthose of the control groups.

The best weight gain of lambs was obtained by administering the RIALbiopreparation mixed with concentrated feeds from the age of one month.In this case, the weight gain of lambs' body weight was 0.8-1 kg higherthan that in the group of control animals.

Survival rate of lambs in experimental groups was higher than that ofcontrol animals.

In experiments on rams with normal and low sexual potency, the sexualactivity of sires, quantitative and qualitative indices of their spermproduct were examined ten-daily in the course of the experiment,prolonging the time to one month after completing the administration ofRIAL to rams.

It has been established that administration of the RIAL biopreparationin the doses of 10 and 30 mg/kg of body weight to tupping rams with anormal sexual potency curtails the time for obtaining the ejaculate by44.0% and 61.18% whereas the reduction of time in the control groupamounts only to 33% due to physiological treatment of animals.

The reduction of time required for ejaculation in the group of rams witha low sexual potency is 49% while it stays unchanged in the controlgroup.

40-60 days after administration of the RIAL, the concentration ofspermatozoons in the ejaculate grows by 12-15%.

EXAMPLE 2 Effect of RIAL on Working Capacity and PhysiologicalParameters of Trotting Horses Participating in Hippodrome Races

Experiments have been conducted in the course of 4 months (June toOctober) with the horses of Russian and American trotting breeds on theMoscow Central Hippodrome.

The conditions of keeping, feeding and care of the experimental horsescomplied fully with the zootechnical norms and veterinary-sanitaryrequirements.

All horses were put under ordinary training loads and were tested inaccordance with the training and racing system recommended by the VNIIK(All-Union Research Institute of Horse Breeding).

Within the entire period of tests, the experimental horses were underconstant veterinary control and their behavior in box stalls and duringtraining were systematically observed.

The RIAL biopreparation was administered to each horse individually atdinner time at ten-daily intervals in a dose of 30 mg/kg of body weight.

The average briskness of horses on all heats within the testing time wasfound by summing up the brisknesses of all heats within the testingperiod and dividing them by the number of heats within the testingperiod.

The "success percentage" and distance parameters were calculated by theformula: ##EQU1##

The effect of administering the RIAL biopreparation on working capacityof horses is given in Tables 5 and 6.

                  TABLE 5                                                         ______________________________________                                        Characteristics of Experimental (E) and                                       Control (C) Groups of Horses                                                                    Average best brisk-                                                                         Winnings at                                                     ness at beginning                                                                           beginning of                                          No. of    of experiment,                                                                              experiment,                                   Group   horses    min.s         rbl                                           ______________________________________                                        E       15        2.20.19       884-85                                        C       15        2.19.63       993-15                                        ______________________________________                                    

In the opinion of horse trainers, the horses which received RIALpreparation have preserved their stamina throughout the entire distanceof the heat. They do not loose the running tempo at the last quarter ofthe round which often is the case with control horses. This opinion canbe confirmed by calculating distance parameters from the followingformula: ##EQU2## where α--distance parameter

∂ν--distance of races won in group

N--number of horses in a group ##EQU3##

                  TABLE 6                                                         ______________________________________                                        Working Capacity Parameters of Experimental (E)                               and Control (C) Horses within Testing Period                                                Impro-  Aver.                                                         Aver.   vement  brisk-                                                        best    of own  ness  Aver.                                                   brisk-  record, of all                                                                              win-   Aver. %                                                                              Distance                                  ness,   aver.,  heats,                                                                              ning,  of     para-                               Group min.,s  s       min.,s                                                                              points success                                                                              meters                              ______________________________________                                        E     2.15.0  5.20    2.17.23                                                                             1071-27                                                                              29.27  1600                                C     2.17.03 2.6     2.18.82                                                                             810-23 21.73   640                                ______________________________________                                    

The average briskness of the horses which received a course of RIAL was2.17.23 while that of control horses within the testing period was2.18.82.

It can be seen from Tables 5 and 6 that administering the RIALpreparation exerts a favorable effect on the horses which undergotraining and suffer heavy physical loads in stress situations.

Thus, the use of RIAL discloses the potential working capacity of aparticular specimen which raises substantially its chances to join thepedigree stock.

The results of blood tests of control and experimental horses aresummarized in Table 7.

                                      TABLE 7                                     __________________________________________________________________________    Results of Horse blood sample analysis                                                     Sample series                                                    Parameter                                                                              Group                                                                             I      II     III    IV     V                                    __________________________________________________________________________    Bactericidal                                                                           E   14.07 + 9.47                                                                         13.88 + 9.87                                                                         37.60 + 5.93                                                                         39.27 + 7.93                                                                         53.07 + 2.23                         activity C   21.97 + 9.81                                                                         24.27 + 5.51                                                                         36.97 + 8.59                                                                         32.29 + 7.71                                                                         50.59 + 5.85                         Lysozyme E   2.58 + 0.72                                                                           3.89 + 2.24                                                                          3.74 + 0.98                                                                          3.94 + 0.82                                                                          4.01 + 0.88                         activity C   3.19 + 0.94                                                                           3.83 + 1.55                                                                          4.90 + 0.84                                                                          3.76 + 0.88                                                                          3.36 + 0.73                                      Fractional composition of blood serum proteins, %                Gamma-globulin                                                                         E   17.45 + 4.96                                                                         20.55 + 3.36                                                                         22.08 + 4.69                                                                         24.46 + 4.55                                                                         24.69 + 3.14                                  C   20.17 + 12.57                                                                        23.27 + 4.12                                                                         19.71 + 2.98                                                                         22.72 + 7.10                                                                         22.93 + 3.40                         __________________________________________________________________________

In all series of analyses, the content of gammaglobulin in experimentalanimals is larger than that in the control animals while staying withinthe limits of the physiological norm which gives ground to asubstantiated conclusion about the stimulating effect of the RIALbiopreparation on synthesis of immunoglobulins and on increasing thelevel of natural resistance of horses.

The data on determining the activity of lysozyme in blood serum ofhorses characterize the state of non-specific resistance of animals. Ascan be seen from Table 7, there was a tendency for an increase in thelyzosyme activity in the blood serum of the experimental group ofhorses.

The bactericidal activity of blood serum also reflects the level ofnon-specific resistance. This parameter depends to a considerable extenton the condition of an animal, season of the year, conditions of work,feeding, etc.

The horses of the control group had a sufficiently high immonologicalstatus with respect to this parameter.

In the first and second months of observation, the resistance ofexperimental horses was less pronounced. However, already by the thirdmonth this parameter in the experimental horses was higher than that inthe control group. Later on, this tendency stays to the end of theexperiment with steady aftereffect of the RIAL preparation (one monthafter the end of RIAL administration the bactericidal activity index was53.07% in the experimental group and 50.59% in the control one).

The use of the RIAL biopreparation in the ration of horses produced thefollowing results:

briskness of experimental horses improved by 2.6 s as compared withcontrol animals;

"success percentage" of experimental horses increased by 8.24% ascompared with control animals;

number of points won by experimental horses increased by 261.04 ascompared with control animals;

experimental horses stayed in good sporting form throughout the racingseason;

improved endurance of horses.

The RIAL biopreparation:

maintains the content of proteins in blood serum at a sufficiently highlevel (within the limits of physiological norm);

exerts a stimulating effect on the synthesis of gamma-globulins therebyraising the resistance of animals;

reduces the concentration of nitrous substances and lactic acid in bloodof horses which testifies to a favorable effect of the preparation onprotein and energy metabolism;

influences the non-specific resistance of the organism, increasing thelysozyme and bactericidal activity of blood serum in horses;

maintains concentration of hemoglobin in blood within the limits ofphysiological norm thus improving the working capacity and endurance ofhorses.

EXAMPLE 3 Determining Efficiency of the RIAL Biopreparation in PigBreeding

Three scientific-economic experiments on the pigs of a new Russian meatbreed CM-1 were conducted in the State Farm "Snezhny" of Magadan region.

In experiment I, the effect of various preparation doses on reproductiveproperties of sows were determined (Table 8).

                  TABLE 8                                                         ______________________________________                                        Experiment I                                                                                     Dose of RIAL per                                                              1 kg of body  Feeding                                      Group    No. of pigs                                                                             weight, mg    conditions                                   ______________________________________                                        I control                                                                              15         0            --                                           II exper.                                                                              16        10            daily from first                                                              day of coupling                                                               to weaning of                                                                 piglets                                      III exper.                                                                             16        25            daily from first                                                              day of coupling                                                               to weaning of                                                                 piglets                                      IV exper.                                                                              18        40            daily from first                                                              day of coupling                                                               to weaning of                                                                 piglets                                      ______________________________________                                    

Experiments II and III (Tables 9 and 10) were devoted to studying theeffect of different RIAL doses on fattening of piglets.

                  TABLE 9                                                         ______________________________________                                        Experiment II                                                                                 Dose of RIAL                                                                  per 1 kg of Feeding                                                   No. of  body weight,                                                                              condi- Feeding period,                            Group   piglets mg          tions  days                                       ______________________________________                                        I control                                                                             22       0          --      0                                         II exper.                                                                             20      10          daily  30                                         III exper.                                                                            22      30          "      30                                         IV exper.                                                                             21      50          "      30                                         ______________________________________                                    

                  TABLE 10                                                        ______________________________________                                        EXPERIMENT III                                                                                Dose of RIAL                                                                  per 1 kg of Feeding                                                   No. of  body weight,                                                                              condi- Feeding period,                            Group   piglets mg          tions  days                                       ______________________________________                                        I control                                                                             22       0          --      0                                         II exper.                                                                             21      50          daily  30                                         III exper.                                                                            21      50          every  15                                                                     other                                                                         day                                               ______________________________________                                    

The efficiency of the RIAL biopreparation was determined by comparingthe results in control and experimental animals and expressing thedifference in percent. All the obtained data were processedbiometrically and appear in Table 11.

                  TABLE 11                                                        ______________________________________                                        Differences Expressed in Percent to Control (I)                               Group ("+" and "-" stand for improved and                                     negative results, respectively)                                                              Group                                                          Parameter        I      II       III  IV                                      ______________________________________                                        Experiment I (reproductive abilities)                                         Multiple pregnancy                                                                             100     +5       +7   +5                                     Macrofetation    100    +44      +67  +67                                     Lactoscence      100    +23      +23  +27                                     2-month piglets:                                                              number of piglets                                                                              100    +21      +31  +28                                     mass of one piglet                                                                             100     +9      +14  +16                                     weight of litter 100    +31      +47  +45                                     Survival rate from birth to                                                                    100    +14      +21  +21                                     weaning                                                                       Average of 7 parameters                                                                        100    +21      +30  +28                                     Experiment II (fattening and meat characteristics                             obtained after administering different doses of RIAL)                         Age of reaching 100-kg                                                                         100     +3      +13  +17                                     weight                                                                        Weight gain during fattening                                                                   100     +5      +23  +32                                     Fodder consumption                                                                             100     +4       +8  +12                                     Average of 3 parameters                                                                        100     +4      +15  +20                                     Thickness of lard                                                                              100     +6      +10  +14                                     Experiment III (different-methods of feeding RIAL)                            Age of reaching 100-kg                                                                         100    +13      +12                                          weight           100    +24      +21                                                           100     +9      +10                                                           100    +15      +14                                                           100    +13      +10                                          ______________________________________                                    

It follows from Table 11 that the RIAL preparation used as an additionto the rations of sows and piglets is extremely effective. A dose of10-40 mg/kg and 10-50 mg/kg of body weight added to the ration of sowsand gilts, respectively, # improves the productivity parameters incomparison with the animals of the control group.

Multiple pregnancy of sows increases by 5-7%, macrofetation, by 44-67%,and lactoscence, by 23-27%. At two months of age of weaned piglets, thenumber of piglets in a litter increases by 21-31%, the mass of onepiglet by 9-16%, and the weight of litter by 14-21% as compared withcontrol animals.

The age of fattening piglets reaching a 100-kg body weight is reduced by3-17%, the average daily gains are increased by 5-31%, and fodderconsumption is reduced by 4-12%. The thickness of lard above the 6-7thoracic vertebra is 6-14% smaller than that of the control animals.

The optimum doses of the RIAL preparation are 25-40 mg/kg of body weightof a sow and 30-50 mg/kg of a gilt.

The most rational method for fattening young pigs is administering theRIAL preparation for a month, every other day, beginning from the firstday of fattening.

EXAMPLE 4 Determining Efficiency of RIAL Biopreparation in PoultryFarming

Investigations have been carried out on cage-kept broiler chickens(cockerels and pullets) of "Hybro-6" cross in groups of 50. Duration ofthe experiment was two months. The keeping conditions satisfied therecommendations in effect. The broiler chickens and laying hens weregiven full-ration formula feeds.

The results of investigations are summarized in the following tables.

                  TABLE 12                                                        ______________________________________                                        Consumption of Fodder per 1 kg of Weight Gain                                         RIAL addi-            Fodder con-                                             tions,     Duration of                                                                              sumption % of                                           mg/kg of   experiment,                                                                              per 1 kg of                                                                            control                                Group   body weight                                                                              days       weight gain                                                                            group                                  ______________________________________                                        Experiment 1                                                                  1 contr.                                                                              --         49         2.36     100                                    2 exper.                                                                              30         49         2.21     93.6                                   3 exper.                                                                              50         49         2.25     95.3                                   Experiment 2                                                                  1 contr.                                                                              --         49         2.45    100                                     2 exper.                                                                              30-50      28         2.36    96.3                                    3 exper.                                                                              30-50      49         2.36    96.3                                    ______________________________________                                    

It follows from Table 12 that fodder consumption per 1 kg of weight gainin experimental groups is lower than it is in the control group.

A higher difference in fodder consumption is registered in case of thelow nutrient values of formula feeds due to better digestibility and theuse of nutrient substances of fodder.

Table 13 summarizes the data on the effect of RIAL additions on the bodyweight of chickens.

                  TABLE 13                                                        ______________________________________                                        Average Body Weight of Cockerels and Pullets, g                               Weight in            Weight in                                                4 weeks              7 weeks        Body weight                               Group   g       %        g     %      gain, g                                 ______________________________________                                        1 contr.                                                                              694.8   100      1550.8                                                                              100    30.9                                    2 exper.                                                                              741.2   106.6    1642.1                                                                              105.8  32.8                                    3 exper.                                                                              719.98  103.5    1618.3                                                                              104.3  32.3                                    ______________________________________                                    

RIAL additions to standard formula feeds given to chickens in doses of30 and 50 mg/kg of body weight have contributed to increased weight gainof broilers by 3.5-6.6% at the age of 4 weeks and by 4.3-5.8% at 7 weeksof age.

The use of RIAL in feeding energetically low nutrient formula feeds hascontributed to weight gain of control chickens by 3.0-6.6% after 4 weeksand by 2.8-7.4% after 7 weeks.

All groups have demonstrated a positive effect when RIAL was used inlow-nutrient feed mixture. In this case, the body weight of experimentalchickens has reached that of the control chickens when the latter havereceived a normal energetically-balanced ration which points to theexpediency of using the RIAL biopreparation in low-nutrient rations ofpoultry.

The investigation of meat quality of chickens has proved that the meatof experimental chickens is noted for a high quality and features atendency to a higher (by 2.2%) yield of edible parts in carcasses.

The chemical composition of meat is within the norm for the given agebracket of chickens.

Introduction of the RIAL biopreparation into the rations of laying hensat the rate of 30 and 100 mg/kg of body weight raises their productivityby 1.9%. The consumption of fodder per 10 eggs is reduced by 2.62%.

The incubating properties of eggs and hatching of chickens in all groupshave improved. The chemical composition of eggs has been within thelimits of the physiological norm.

EXAMPLE 5 Determining Efficiency of RIAL Biopreparation in Apiculture

The efficiency of RIAL doses has been determined by the effect of thepreparation on bees in laboratory conditions. To produce uniage bees,honeycombs with mature bee brood have been taken from a bee colony,placed into single-frame isolators and transferred into thermostat TPS-3at a temperature of 34° C. and a relative air humidity of 70%. Groups of50 bees at the age of up to 24 hours have been placed intoenthomological live boxes and kept at 28° C. Every three days they havebeen given sugar syrup with RIAL.

The number of perished bees was registered daily in the live boxes. Inaddition, the condition of middle and hind intestines and consistency offecal matter were inspected in experimental bees.

15 doses of the RIAL biopreparation were tested, three times each, in 48live boxes. The results of the experiment appear in Table 14 whichdemonstrates that the bees receiving sugar syrup with 50 mg/l of RIALlived for 21 days which is three days longer than the control bees. Thebees of this group had the smallest filling of hind intestine withfeces.

                  TABLE 14                                                        ______________________________________                                        Effect of RIAL on Life Span of Bees and                                       Condition of Their Hind Intestine                                                       On the 9th day of test, n = 20                                             Life     aver. wt of                                                          span,    hind intes-                                                   Dose of                                                                              days,    tine with                                                     RIAL,  n =      contents, condition of hind                                   mg/kg  150      mg/bee    intestine, points                                   ______________________________________                                        10-20  15       30        2   Intestine wall loose,                                                         filled with liquid,                                                           homogeneous, easily                                                           spreading excrements,                                                         only partly removable                                                         from belly                                      30-40  14       20        2                                                   50     21       14        4-  Intestine retains                                                             fully its structure,                                                          completely and easily                                                         removable, wall flex-                                                         sible, non-rupturing,                                                         holds excrements well:                                                        muscles clearly seen                                                          with tracheas in the                                                          form of white threads                            60-140                                                                              14       22        2                                                   150    14       24        3-  Intestine completely                                   All bees               removable with non-                                    perished               rupturing walls filled with                            at time                slowly spreading homo-                                                        geneous excrements                              Contr. 18       20        2                                                   ______________________________________                                    

The shortest life span (6 days) was observed in bees given syrup with 30mg/l addition of RIAL. The life span of bees in live boxes given otherdoses of the preparation was 14-15 days.

It should be noted that the bees fed with the preparation in 150 mg/l ofsyrup exhibited high mobility up to the 14th day and then they perished.

The best dose of the preparation--50 mg/l--and a doubtful dose--150mg/l--have been tested on bee colonies in the bee growing period, i.e.in July-August.

The test involved counting the amount of brood and honey and formingthree 10-colony groups analogous in vitality, amount of brood and honey.All colonies were given a liter of sugar syrup three times at three-dayintervals. The first (control) group received syrup without additions,the second one, syrup with a 50 mg/l dose and the third group, syrupwith a 140 mg/l dose.

Within three periods after supplementations, each group of coloniesreceiving syrup with 50 mg/l and 150 mg/l of the preparation has grownon the average 23 and 21 thousand specimens, respectively, while in thecontrol group the result was 17.7 thousand specimens.

The optimum dose of the RIAL addition to sugar syrup for feeding-up thebees was found to be 50 mg/l.

At the bee growing time in spring and in August, the bee colonies may begiven stimulating supplements with 50 and 150 mg/l doses of thebiopreparation.

To determine the effect of the RIAL biopreparation on the winteringbees, the colonies were kept in simulated wintering conditions inclimatic chambers at 6° C. and a relative air humidity of 72%.

For this purpose the test consisted in picking up eight bee colonies,removing the brood from them on the June 11th, reducing the number ofbeeways to 7-8 and feeding each colony with 7 l of syrup. Four coloniesreceived syrup with the best dose of RIAL, i.e. 50 g/l, while the otherfour, sugar syrup without any additions. On the 5th of June, thecolonies were placed into climatic chambers where they stayed toSeptember 15.

In the middle of June and September, samples of bees were taken fordetermining some of their physiological parameters.

It was found that mortality of bee colonies feeding on sugar syrup withRIAL addition in the climatic chamber was two beeways, while in thecontrol colonies it was 0.5 beeways higher. The control colonies used0.3 kg more feed with the resultant increase in excrement load.

The catalase of rectal glands in the bees of experimental group provedto be higher which is an evidence that the use of the RIALbiopreparation improves the winter hardiness of bee colonies.

Thus, the tests have demonstrated that feeding the bees with theaddition of the RIAL preparation (50 mg/l of sugar syrup) in any beegarden counting from 100 bee colonies with population in May varyingfrom 3.5 to 5 beeways makes it possible to grow 30 additional beepackages. In honeyproducing bee gardens every 100 colonies can produceadditionally 550 kg of honey.

INDUSTRIAL APPLICABILITY

The present group of inventions can be used to advantage in animalbreeding, fur farming, poultry farming and apiculture for feedingvarious groups of agricultural animals, fowl and bees.

We claim:
 1. A biopreparation comprising the following in terms ofweight percent:

    ______________________________________                                        Amino Acids                                                                   Free                 30-40                                                    Fixed                30-55                                                    Lipids               0.01-2                                                   Macronutrients                                                                Fe, Ca, Na, K, P, Mg, S, Cl                                                                        2-6                                                      Microelements                                                                 Mn, Zn, Cu, Sb, As, Sn, Mo, Bi                                                                     0.5-1.5                                                  Compounds of non-metals,                                                                            8-12                                                    excluding monosugar                                                           Carbohydrates        0.001-0.1                                                Moisture               4-10.                                                  ______________________________________                                    


2. A method for feeding an animal involving introduction of abiopreparation into its ration CHARACTERIZED in that the biopreparationis the biopreparation of claim
 1. 3. The method of claim 2 CHARACTERIZEDin that the biopreparation is introduced into the ration of poultry in adose of 330-625 g/t of feed.
 4. The method of claim 2 CHARACTERIZED inthat the biopreparation is used in apiculture by adding it to sugarsyrup in a dose of 50-150 mg/l of syrup.
 5. The method of claim 2 inwhich the animal is poultry.
 6. The method of claim 2 in which theanimal is a bee.
 7. The method of claim 2 in which the animal is asheep.
 8. The method of claim 2 in which the animal is a horse.
 9. Themethod of claim 2 in which the animal is a swine.